Setting the standard in small-volume filtration (1-200 mL), the unsurpassed consistency of Millex ® syringe filters in sample preparation has led to the development of many methods specifying their use. Featuring reduced sample loss due to minimal hold-up volumes, Millex ® syringe filters are ideal for use in a variety of applications including:
This syringe filter is made with GHP (hydrophilic polypropylene) membrane for low protein binding and features a polypropylene housing with female Luer-lock inlet and standard male slip Luer outlet connections. The membrane is HPLC certified for low levels of UV-absorbing extractables. Membrane: GHP Membrane. Pore Size: 0.45 µm. Typical hold-up volume: \\<100µL. Pall Life Sciences #: 4560.
Aijiren Techbrand Syringe Filters - Sterile:Filters and Filtration . Low hold-up volume helps you to recover virtually all of your sample after filtering. Tested for 100% membrane and housing integrity, eliminating concerns about fluid loss. Individually wrapped. Hydrophilic PVDF Membrane.
To differentiate between moderately and highly active vaccines, some studies may use a dose range including lower or higher challenges. In our hands, effective DNA vaccines can provide 100 % protection at up to 500 × LD 50 . Some investigators use a two-dimensional matrix in which both vaccine dose and HSV-2 challenge dose are independently
Due to the interdependence of the various areas or areas that make up a sterile radiopharmaceutical processing facility, it is essential to define and control the dynamic interactions permitted between areas. When designing doors, consider the placement of door closures, door surfaces, and the movement of the door, all of which can affect airflow.
Hold-Up Volume Another aspect of choosing the right filter size is the hold-up volume. This is the volume of liquid remaining in the filter after use. A filter with a low hold-up volume is recommended for use with expensive fluids or those with limited availability.
For small sample volumes, sterile syringe filters are available. Sterile syringe filters have a luer lock inlet that will connect directly onto a syringe for easy filtration of your sample. These devices can rapidly filter small volumes and are available with diameters of 13, 25, 32, or 37 mm. Mid-volume Samples
Nov 11, 2022 · Immunocompromised populations are highly vulnerable to developing life-threatening infections. Strategies to protect patients with weak immune responses are urgently needed. Employing trained immunity, whereby innate leukocytes undergo reprogramming upon exposure to a microbial product and respond more robustly to subsequent infection, is a promising approach. Previously, we demonstrated that
Designed for sterile and nonsterile small-volume filtration (1-200 mL) Produced with high-quality Millipore ® membranes; Available in 4, 13, 25, 33, and 50 mm diameters; Minimal hold-up volume reduces sample loss; Low extractable and low binding membrane options; Whatman ® syringe filters
Filter Size 25 mm (1) 32 mm (2) Hold-Up Volume <300 µL (5) <60 µL (3) <100ul (2) <650 ul (1) Membrane Brand Emflon II (1) Membrane Material Supor (Hydrophilic polyethersulfone) (16) Versapor (acrylic copolymer on a non-woven support) (3) Fluorodyne II membrane (hydrophilic PVDF) (1) Nylon (1) Posidyne (+ Nylon) (1) Show all Pore Size 0.1 µm (2)
Mix samples (93.5 μL total volume) by pipetting up and down. i. Quick spin to collect all liquid from the sides of the tube. c. Incubate 15 min at 20°C in a Thermomixer. d. Add 3 μL USER Enzyme (provided with the kit) to the ligation mixture resulting in a total volume of 96.5 μL. i. Mix well by pipetting up and down. e.
sterile pall ghp lower hold-up volume Pall Minispike Acrodisc Syringe Filters, Pall Life Sciences Combining top-notch and uncompromising quality with an affordable price, the Pall Minispike Acrodisc Syringe Filters, Pall Life Sciences Ghp Membrane 4567/ 28139-554 can fulfill your laboratory needs while still offering a great value for the money.
With the index finger on the clavicle and distributing some pressure to hold the jugular vein cranially, and placing the thumb on the jawline, insert a 25-G, 5/8-inch needle attached to a syringe (1-ml or 3-ml depending on volume required), with needle bevel side up, into the skin at a slight ∼10-25° angle to the skin (Fig. 9).
During filtration, all systems retain some liquid that cannot be recovered. This is called “hold-up” volume. The larger the hold-up volume, the greater the loss of media and reagents. However, the Upstream “Hold up” Volume can be recovered by pumping air through the vent. A hefty percentage of the upstream volume can be collected from the process.
Stericup Quick Release-GP Sterile Vacuum Filtration System, 250 mL process volume, 0.22 µm pore size, polyethersulfone membrane, pkg of 12, radio-sterilized Expand S2GPT05RE Steritop Quick Release, Sterile 500 mL Steritop Quick Release-GP 45 mm threaded bottle top filter with 0.22 µm pore size fast flow polyethersulfone (PES) Express PLUS membrane.