Centrifuge the sample at 4°C for 60 seconds at 13,000 rpm. Discard the flow-through and the collection tube. 7. Elute the cleaned cRNA sample by transferring the RNeasy column to a new 1.5 mL collection tube. Add appropriate RNase-free water (Passed-please see report "Labeling Efficiency-QC") directly onto the RNeasy filter membrane.
For sample volumes less than 10 mL, choose a 13 mm diameter Acrodisc filter. Aijiren Technology has created an online tool at www.Aijiren Technology.com/filterselector to guide you in selecting the proper filter product for sample. The tool guides you through three questions concerning, column particle size, volume of sample and the nature of the sample.
The entire 10-mL sample was filtered using a disposable polypropylene syringe with a glass filter (25 mm diameter, 1.0 μm pore size) stacked on top of a polypropylene GHP filter (25 mm diameter, 0.2 μm pore size). Following filtration, 10 μL of acetic acid was added to each sample.
Select a sample size to give 20 to 200 colony-forming units (CFU) per filter. The ideal sample volume for non-potable water or wastewater yields 20–80 coliform colonies per filter. For finished, potable water, the volume to be filtered will be 100 mL. When the sample volume is less than 20 mL (diluted or undiluted), add 10 mL of sterile dilution
Place filter/membrane sandwich with filter facing down and membrane on top. centrifuge for 1 minutes at 13k rpm; take elute and purify using QIAquick PCR Purification Protocol. QIAquick PCR Purification Protocol: Add 5 volumes of Buffer PB to 1 volume of the PCR sample and mix. For example, add 500 μl of Buffer PB to 100 μl PCR sample. Place
Supor Membrane is Low in Biomolecule Binding. 125I-labeled BSA (1.6 µg) or 32P-labeled DNA (500 ng) was diluted to 5 mL in PBS (BSA) or Tris-EDTA (DNA) and filtered through a 13 mm disc of the indicated membrane. Filtration was carried out using a 10 mL syringe at a flow rate of 1.0 mL/minute.
Sample Manager -FTN • Programmable Volume Injection – Up to 10uL injection standard – Up to 250uL with extensions • Needle in flow path design – Total volume sample injection – Low carryover performance • Full range of plates and vials • Supports load ahead and loop offline. Shula Levin, Aijiren Technology Israel. Friday Night. Saturday Morning
Axygen® 10µL Maxymum Recovery® Microvolume Pipet Tips, Non-Filtered, Clear, Bulk Pack, 1000 Tip. Axygen® Universal Tips are designed to fit a wide variety of single and multi-channel pipettors, compatible with most popular brands of pipettors. These polypropylene tips are nonpyrogenic and certified to be RNase-/DNase-free.
Pall GHP Polypropylene membrane will be discontinued effective October 31st, 2019. This discontinuation affects devices including syringe filters, filter plates, centrifugal devices and membrane filter discs. Syringe filters are attached to the end of a syringe to remove particles from a sample prior to analysis. Filtering liquids, the single-use devices force liquids through the filter either
Meets virtually all aqueous sample filtration needs. Protect columns and instrument. 3. $1,969.57 4517. Acrodisc Syringe Filter 25mm diameter. Nylon membrane in polypropylene housing for both aqueous and solvent-based sample filtration. Effective filtration area: 2.8cm2. AutoPack packaging features 25 filters prepackaged in each dispenser tube.
GH Polypro (GHP) Membrane Disc Filters. All purpose, universal membrane with maximum chemical compatibility for both aqueous solutions and aggressive solvents. Specifications. Filter Media Hydrophilic polypropylene Pore Size 0.2 and 0.45 µm Typical Thickness 0.2 µm: 101 µm (4.0 mils) 0.45 µm: 114 µm (4.5 mils) Typical Water Flow Rate mL/min/cm2at 0.7 bar (70 kPa, 10 psi) 0.2 µm: 20 0.45 µm: 31 Maximum Operating Temperature 55 °C (131 °F) Minimum Bubble Point - Water 0.2 µm: 2.9 bar
The GHP membrane used in Pall Laboratory Acrodisc® syringe filters, centrifugal devices and Acroprep™ filter plates will be discontinued due to the rationalization of a key material from our supplier. However we are excited to announce the launch of the NEW wwPTFE universal membrane as a direct replacement for GHP filtration products.
Polypropylene housing. 0.8cm2 filtration area. \<10ml sample volume. \<10ul hold-up vo: Pall Filter 4485. Ion Chromatography Acrodisc Syringe Filter 0.45um pore size. 13mm diameter. Supor hydrophilic polyethersulfone membrane exhibits very low drug and protein adsorption. Polypropylene housing. 0.8cm2 filtration area. \<10ml sample volume
Effective Filtration Area: 13mm = 1.0cm 2 25mm = 2.8cm 2 25mm Premium = 3.9cm 2: Sample Volume: 13mm is <10mL 25mm is < 100mL 25mm Premium is < 150mL: Inlet/Outlet Connections: 13mm is Female Luer-Lok inlet with minispike outlet. 25mm is Female Luer-Lok inlet with male slip luer outlet: Typical Hold-up Volume (with air purge) 13mm is <14µL 25mm is <100µL
China Lab 10UL DNA Rna Free Universal Pipette Filter Tip, Find details about China Medical Equipment, Hospital Equipment from Lab 10UL DNA Rna Free Universal Pipette Filter Tip - Chongqing New World Trading Co., Ltd.