Fast flow and low binding for cell culture media preparation with Millipore Express ® PLUS polyethersulfone (PES) membrane; Durapore ® PVDF membrane is the lowest binding membrane for protein-rich solutions; Many are CE-marked and/or approved as medical devices, meaning that they can be used to filter pharmaceutical solutions
Membrane Filter Materials Mixed Cellulose Esters (MCE) Mixed Cellulose Esters is a very low protein binding membrane that is ideal for aqueous based samples. MCE membranes are an excellent choice when maximum protein recovery in the filtrate is critical. Laboratory studies show that MCE membranes bind less protein than PVDF or Polysulfone
The Pall GH Polypro (GHP) hydrophilic polypropylene membrane offers maximum chemical compatibility. Pall GHP membrane offers low protein binding (96.5% recovery), and low extractables. Pall GHP Nanosep Centrifuge Devices can be spun at 14,000g with leak-free operation. Pall Jumbosep Centrifuge Devices, Pall Centrifuge Devices
Bio-Rad's Sequi-Blot PVDF membrane delivers outstanding performance in protein sequencing, providing the binding capacity to sequence even low-abundance samples. Nitrocellulose Membrane, 0.45 µm Bio-Rad's nitrocellulose is a proven medium for western, northern, and Southern blotting.
For aqueous samples, it is preferable to use a hydrophilic membrane (GHP, PVDF or Nylon) versus a hydrophobic membrane (PTFE). With proteinaceous samples, a low protein binding filter (GHP or PVDF) is preferable to other hydrophilic filters (Nylon).
To recover analytes or other valuable components of interest after filtration, it is important to choose a filter with low nonspecific binding. For example, hydrophilic PVDF microporous membrane was found to be extremely inert and has both the lowest protein binding properties and the highest product recovery.
Low protein binding membrane gives high recovery of critical proteinaceous samples; AutoPack™ packaging is easy to use and the Automation Preferred™ certification assures you of smooth operation and worry-free performance 24 hours a day in automated workstations; Accurate analysis. HPLC certified for low levels of UV-absorbing extractables See full list on mgscientific.com
Polyvinylidene Fluoride (PVDF) and hydrophilic PVDF – Low protein binding; Regenerated Cellulose (RC) – Very low protein binding; Anopore – Low protein binding. Additional Syringe Filter Considerations As you can see, there are numerous options for low protein binding syringe filters, so other factors need to be taken into consideration. One key consideration is application or how you plan on using the filter. This chart can help you narrow your choices of low protein binding syringe
AcroPrep™ 384-well filter plates with GHP membrane 74 • Similarly, the highest levels of protein binding are also found on PVDF membranes. These mem-
Acrodisc 25mm Premium Syringe Filter GxF borosilicate glass prefilter. Polypropylene housing. 0.2um GHP hydrophilic polypropylene membrane for chemical compatibility fast flow rates low protein binding low levels of UV-absorbing extractables. Filtration area: 3.9cm2. Hold-up volume: \<125uL. 9. $2,737.93 AP-4307
This data highlights the fact that protein binding studies using static soak (Table 2) or recircularization do not represent the binding of proteins under typical conditions. While most microfiltration membranes tested had very low binding, Versapor® membrane, a general-purpose clarification membrane, bound significant amounts of protein.
cause loss of significant amounts of biological materials due to unwanted protein binding with the membrane or introduce unexpected interferences to the samples from the syringe filter. Therefore, low protein binding and cleanliness are important features of a syringe filter’s performance. Polyethersulfone (PES) and polyvinylidene
SmartSite™ extension set, 0.2 micron filter (1.72 sq. in low protein binding), pinch clamp(s) Extension set 0.2 micron filter (1.72 sq. in low protein binding) pinch clamp(s) 1 SmartSite™ needle-free valve(s) 6" from spin male luer lock. Not made with DEHP. L: 18 in L: 46 cm PV: 5 mL fluid path sterile.
MS® PES is an inherently hydrophilic membrane whose wettability has been proved extraordinary strong comparing to other membrane and that leads to its high throughput with fast flow rates. PES membrane also shows low protein binding. The membrane can be broadly utilized in sample preparation, sterile filtration and transfusion treatment.
Acrodisc® Syringe Filters. Pall Laboratory. of filter media: hydrophilic nylon membrane for excellent chemical compatibility with esters, bases, and alcohols, without pre-wetting; GHP (hydrophilic polypropylene) membrane for low protein binding; and hydrophilic PVDF (polyvinylidine fluoride) membrane for use in aqueous and non-aggressive,….